Journal: BMC Developmental Biology

Article Title: BMP-SMAD signalling output is highly regionalized in cardiovascular and lymphatic endothelial networks

doi: 10.1186/s12861-016-0133-x

Figure Lengend Snippet: The GFP reporter protein faithfully recapitulates the transcriptional activation of the BRE :: gfp transgene. In situ hybridisation for gfp mRNA ( red ) and direct GFP fluorescence ( green ) on cryosections through the cardinal vein ( a – d ) and the ventricular trabeculae ( e – g ) at E11.5. DAPI is used to stain nuclei. The boxed area in ( a ) is enlarged in panels ( b – d ). The corresponding low magnification view of the trabeculae in the ventricle is not provided because of insufficient intensity of the ISH signal. White arrowheads indicate ECs double positive for gfp mRNA and direct GFP fluorescence. The red and green arrows depict ECs only positive for gfp mRNA or GFP fluorescence respectively. Scale bars: 100 μm ( a ); 50 μm ( b – d ); 10 μm ( e – g )

Article Snippet: The GFP fluorescent in situ hybridisation (ISH) probe was custom designed with the probe designer tool from Stellaris (LGC biosearch technologies).

Techniques: Activation Assay, In Situ, Hybridization, Fluorescence, Staining

Journal: PLoS ONE

Article Title: Wdr18 Is Required for Kupffer's Vesicle Formation and Regulation of Body Asymmetry in Zebrafish

doi: 10.1371/journal.pone.0023386

Figure Lengend Snippet: (A–F) Embryos were injected at the 1-cell stage with Ca 2+ indicator (Ci) or co-injected with wdr18 MO1. Ca 2+ patterns at the 5- to 8-somite stage were imaged by fluorescence microscopy and the images were converted to an intensity scale (colors from red to green indicate intensity from high to low). (A, B) The autofluorescent image of an un-injected control embryo (B) with its corresponding DIC image (A). (C, D) A control embryo injected with Ca 2+ indicator only, showing fluorescence in the KV with elevated signals on the left side. (E, F) An embryo co-injected with Ca 2+ indicator and wdr18 MO1. The KV is smaller and the fluorescence signal is evenly distributed throughout the KV. nc: notochord. The ratio shown in the lower right corner of B, D and F indicate the number of defective embryos versus total embryos.

Article Snippet: Ca 2+ green-dextran 10,000 MW (Molecular Probes/Invitrogen) was used at 0.05% (w/v) for microinjection into zebrafish embryos at the 1-cell stage with an injection volume of about 0.5 nL.

Techniques: Injection, Fluorescence, Microscopy, Control